Publications

Rapid, Cost - Effective Approach to Human sperm Morphology by using Pre- Stained Methylene Blue Slides

Abstract: Background: Sperm morphology is a crucial aspect of male fertility, impacting reproductive success. This study delves into the evaluation of a simplified and cost-effective staining method for accurate sperm morphology assessment, addressing the challenges associated with conventional techniques. Materials and Methods: This study was conducted at St. John’s Medical College, Bangalore, with three adult male subjects. The methodology included meticulous sample collection, preparation, and staining of prestained methylene blue slides, emphasizing simplicity and rapidity. Results: In this study clear visualization of the sperm morphology was achieved. Notably, the stain facilitated the distinct observation of the sperm structure, including the clear identification of the nucleus, middle piece, and tail. Conclusion: Staining advocates for widespread adoption of this simplified technique, particularly in primary health care settings. The paper contributes to enhancing accessibility and affordability in sperm morphology evaluation.